My excuse for not doing so has been, that there were many exhaustive treatises on this subject already before the public, written by Messrs. Langstroth, Quinby, King, Cook, Root and others-hence there was no reason for thrusting more books upon the world, which had nothing for their subject-matter but the general outlines of bee-keeping. To be sure, there are two little books in pamphlet form bearing my name, viz.: As all bee-keepers of to-day are aware, I have given all of my best thoughts, on the subject which the desired book would cover, free to the world through our many bee-papers, so, had I complied with the request made, the matter in the book would have been mainly that which I had written before; and owing to this self-same cause, the reader will perhaps find some fault with the present work.
Usually the volume in the slot is significantly more than the drops on the side of the pin so the precision of liquid transfer is not effected greatly in liquid to liquid transfers and not at all in liquid to membrane transfers.
One of the phenomena of slotting pins is the great reduction in the hanging drop at the tip of the pin because of the slot. This results in the volume of liquid to be transferred, being close to the volume of the slot. Note that slot pins will not transfer the volume of the slot to a dry well or glass slide but can be made to deliver a larger volume than a hanging drop by causing the pin to strike the surface at a high rate of speed.
This is best achieved when controlled by a robotic system. Because it is relatively easy for us to cut any size of slot we choose, it is simple to make custom Slot Pin Replicators. We have several that we make and stock and they are listed below. If you don't see what you need, call us and we will make a special one for you.
They are also useful in spotting nanoliter volumes onto glass slides.
FP4 slot pins are our newest slot pins. Although they transfer relatively large volumes 1, and 2, ul that could be easily handled by the 1.
Thus companies that have their compound libraries in 1, well microplates find them very useful in High Throughput Screening assays done in the format. The larger slot volume FP6 series floating pins cover the slot volumes from 0. To make slots that will yield the larger volumes we have to use pins of a larger diameter.
The end view of the slot is illustrated in photo to the right.
The 25 ul P11AS25 pins are 4. We can also make custom slot pins that will deliver as little as 6 nl to meet your specific needs.
Also see our 8, 12, 16 and 24 Replicator Strips with slot pins and 8, 12, 16 and 24 Floating Replicator Strips with slot pins. These are good for loading gels and making microarrays on glass slides.
Both of the pins are used to make quantitative liquid to liquid transfers or liquid to membrane blots.Scientific American is the essential guide to the most awe-inspiring advances in science and technology, explaining how they change our understanding of the world and shape our lives.
The advantage of the solid pins is that the hanging drop provides a very accurate and precise method of transferring as little as 2 nanoliter volumes of liquid to membranes or dry microplate wells.
The Metal Magnetic Memory Method - a New Trend in Engineering Diagnostics. Traditional methods and means of diagnostics (ultrasonic inspection, magnetic particle inspection, X-ray) are oriented to detecting the already developed defects and by their designation can not prevent sudden fatigue damages of equipment, which are the main reasons of failures and sources of maintenance staff .
The Bradford protein assay was developed by Marion M. Bradford in It is a quick and accurate spectroscopic analytical procedure used to measure the concentration of protein in a solution. The reaction is dependent on the amino acid composition of the measured protein. Why a Scientific Format?
The scientific format may seem confusing for the beginning science writer due to its rigid structure which is so different from writing in the humanities.
One reason for using this format is that it is a means of efficiently communicating scientific findings to the broad community of scientists in a uniform manner. Waist Measurement Waist size is an additional, independent risk factor and can be used in conjunction with any other method.
It reflects growing evidence that excess visceral fat - surrounding the abdominal organs - on its own increases the chance of heart disease or diabetes.